Flow cytometric analysis of DNA content for ploidy determination in human solid tumors. | Zendy

Bart Barlogie | Zendy; W. Göhde | Zendy; Benjamin Drewinko | Zendy

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Flow cytometric analysis of DNA content for ploidy determination in human solid tumors.

Author(s) -

Bart Barlogie,

W. Göhde,

Benjamin Drewinko

Publication year - 1979

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/27.1.374615

Subject(s) - ethidium bromide , flow cytometry , dna , ploidy , pathology , staining , multiple myeloma , biology , microbiology and biotechnology , chemistry , medicine , immunology , biochemistry , gene

Flow cytometric studies of cellular DNA content were conducted in 26 patients with a variety of neoplasms. Cell dispersal was achieved with pepsin treatment, and a combination of ethidium bromide and mithramycin was used as DNA specific staining procedure. All measurements were conducted with a new sheath flow chamber in a PHYWE ICP 11 pulse cytophotometer. All but one patient with multiple myeloma had unimodal tumor cell DNA distributions. With human granulocytes as reference standard, 24 of 26 tumors were aneuploid; and of these, 23 showed varying degrees of hyperdiploidy. Except for one patient, ploidy abnormalities were stable on repeat examination.

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