Multiparameter kinetic analysis of killer cell initiation by using immune RNA. | Zendy

Peter John Kmieck | Zendy; C. Bruce Bagwell | Zendy; J L Hudson | Zendy; G. L. Irvin | Zendy

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Multiparameter kinetic analysis of killer cell initiation by using immune RNA.

Author(s) -

Peter John Kmieck,

C. Bruce Bagwell,

J L Hudson,

G. L. Irvin

Publication year - 1979

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/27.1.374613

Subject(s) - cytotoxicity , microbiology and biotechnology , cell , thymidine , dna , flow cytometry , rna , chemistry , spleen , cell cycle , immune system , biology , biochemistry , immunology , in vitro , gene

By treating nonsensitized C57BL/6J spleen derived lymphocytes with EL-4 tumor cell directed xenogeneic extracted RNA we were able to monitor early changes in cellular DNA content by flow cytometric (FCM) analysis and 3H-thymidine uptake. These kinetic parameters were correlated with cell mediated cytotoxicity which appeared as early as 8 hr after activation as measured by release of chromium-51 from labeled EL-4 target cells. Flow cytometric analysis and 3H-thymidine uptake data shown peak S phase activity at 72 hr. Maximum cytotoxicity was observed at 48 hr. Cell cycle kinetic parameters were correlated with the appearance of cell mediated cytotoxicity.

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