Multiangle light-scattering analysis of murine teratocarcinoma cells.
Author(s) -
Douglas E. Swartzendruber,
B. Price,
L B Rall
Publication year - 1979
Publication title -
journal of histochemistry and cytochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.971
H-Index - 124
eISSN - 1551-5044
pISSN - 0022-1554
DOI - 10.1177/27.1.374600
Subject(s) - stem cell , yolk sac , embryonic stem cell , p19 cell , microbiology and biotechnology , biology , cellular differentiation , embryoid body , adult stem cell , in vitro , chemistry , embryo , biochemistry , gene
Stem cells of the mouse testicular teratocarcinoma are capable of giving rise in vivo and in vitro to a wide variety of cell and tissue types representative of each embryonic germ layer. Multiangle light-scattering measurements in a flow system have been made on these stem cells and on a variety of their differentiated derivatives. This technique is capable of distinguishing the stem cells from parietal yolk sac cells, visceral yolk sac cells, neuronal cells and squamous cells. However, multipotential stem cells cannot be distinguished from stem cells that are restricted in their development to a single pathway.
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