Imaging in flow.
Author(s) -
David B. Kay,
James L. Cambier,
Leon L. Wheeless
Publication year - 1979
Publication title -
journal of histochemistry and cytochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.971
H-Index - 124
eISSN - 1551-5044
pISSN - 0022-1554
DOI - 10.1177/27.1.374597
Subject(s) - slit , flow (mathematics) , detector , orientation (vector space) , optics , optical imaging , computer science , physics , mechanics , mathematics , geometry
Imaging in flow has been valuable in investigating discrepancies in flow cell measurements due to cell orientation and flow dynamics. This paper discusses optical consideration in flow imaging, slit and full field imaging systems and various cell motion arresting techniques from the standpoint of image plane exposure and suitable detector choices. It concludes with an explanation of the slit-imaging techniques employed in a multidimensional slit-scan flow system and slit-scan correlation system.
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