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A real-time delay monitor for flow-system cell sorters.
Author(s) -
John C. Martin,
Scott R. McLaughlin,
Randy Hiebert
Publication year - 1979
Publication title -
journal of histochemistry and cytochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.971
H-Index - 124
eISSN - 1551-5044
pISSN - 0022-1554
DOI - 10.1177/27.1.374586
Subject(s) - sorting , computer science , flow (mathematics) , point (geometry) , simulation , computer hardware , real time computing , physics , mechanics , mathematics , geometry , programming language
For optimum performance in cell sorting, it is critical to assure proper timing in the charging of droplets to be deflected. A method for determining the transiet delay time in cell sorters has been devised and applied to daily operation in the Los Alamos sorter systems. This delay monitor relies on detection of either scattered or absorbed light from cells in the fluid stream near the point of droplet breakoff.

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