Intracellular pH-determination by fluorescence measurements. | Zendy

Jan Visser | Zendy; A A Jongeling | Zendy; Hans J. Tanke | Zendy

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Intracellular pH-determination by fluorescence measurements.

Author(s) -

Jan Visser,

A A Jongeling,

Hans J. Tanke

Publication year - 1979

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/27.1.35567

Subject(s) - fluorescein , fluorescence , intracellular , flow cytometry , chemistry , intracellular ph , biophysics , excitation , analytical chemistry (journal) , chromatography , microbiology and biotechnology , biochemistry , biology , optics , physics , quantum mechanics

A method was developed to determine the intracellular pH (pHi) of individual cells by use of fluorescence measurements. The method is based on the observation that the fluorescence excitation spectrum of fluorescein is pH-dependent. Fluorescence excitation spectra from individual rat bone marrow cells treated with fluorescein diacetate (FDA) were compared with those of fluorescein solutions of known pH values. Cells which were suspended in media of pH between 4.0 and 8.1 with high to normal buffering capacities had pHi values equal to those of the media. Cells suspended in media with low buffering capacities maintained a pH,i of 6.7 +/- 0.2. Preliminary results indicated that the pHi of individual cells may also be determined by using flow cytometry.

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