Binding of deoxyribonuclease I to actin: a new way to visualize microfilament bundles in nonmuscle cells. | Zendy

E Wang | Zendy; A R Goldberg | Zendy

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Binding of deoxyribonuclease I to actin: a new way to visualize microfilament bundles in nonmuscle cells.

Author(s) -

E Wang,

A R Goldberg

Publication year - 1978

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/26.9.361884

Subject(s) - deoxyribonuclease i , microfilament , actin , fluorescence microscope , microbiology and biotechnology , actina , phalloidin , staining , immunofluorescence , intracellular , biophysics , chemistry , microscopy , fluorescence , biology , cytoskeleton , antibody , cell , pathology , biochemistry , dna , immunology , optics , genetics , medicine , physics , base sequence

Intracellular actin-containing fibers can be visualized by indirect immunofluorescence microscopy when they are stained with antibody directed against DNase I. The location of actin-containing fibers in cells appears to be similar to the staining pattern of antibody to actin. Actin fibers were also visualized by direct fluorescent microscopy with rhodamine-conjugated DNase I.

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