The use of polyethyleneimine for demonstration of anionic sites in basement membranes and collagen fibrils.
Author(s) -
J W Schurer,
D. Kalicharan,
Philip J. Hoedemaeker,
I. Quintus Molenaar
Publication year - 1978
Publication title -
journal of histochemistry and cytochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.971
H-Index - 124
eISSN - 1551-5044
pISSN - 0022-1554
DOI - 10.1177/26.8.690407
Subject(s) - fibril , basement membrane , membrane , cationic polymerization , choroid plexus , collagen fibril , chemistry , biophysics , anatomy , biochemistry , polymer chemistry , biology , central nervous system , neuroscience
Strong cationic polyethyleneimine (PEI) is used to trace anionic sites in lung, choroid plexus, glomeruli, blood vessels and also on collagen fibrils. In the mentioned tissues, PEI is found in the basement membranes with a spacing identical to that found on collagen fibrils. It is assumed that the acid endgroups of tropocollagen molecules are responsible for the binding of PEI.
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