Negative staining of whole cells: transmission electron microscopy of peripheral organelles in rat venous endothelial cells. | Zendy

Darlene K. Racker | Zendy

Open Access

Negative staining of whole cells: transmission electron microscopy of peripheral organelles in rat venous endothelial cells.

Author(s) -

Darlene K. Racker

Publication year - 1978

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/26.5.77873

Subject(s) - organelle , cytoplasm , electron microscope , microbiology and biotechnology , staining , ultrastructure , membrane , negative stain , chemistry , cell , biophysics , transmission electron microscopy , cell membrane , pathology , biology , anatomy , materials science , nanotechnology , biochemistry , medicine , optics , physics

The study of whole negatively stained cells has revealed details of cellular organelles in rat venous endothelial cells. In particular, details of surface membrane organelles and small tubular structures were demonstrated. The surface membrane organelles which appeared "vesicular-like" were found to be connected with small tubular attachments. These findings were correlated with those described by other techniques. It is significant that this simple technique appears to permit the demonstration of fine details of three-dimensional cytoplasmic structures.

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