A detection method for lactic dehydrogenase activity in the inner ear. | Zendy

Takeo Omata | Zendy; Iwao Ohtani | Zendy; Kohsei Ohtsuki | Zendy; Jin OUchi | Zendy

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A detection method for lactic dehydrogenase activity in the inner ear.

Author(s) -

Takeo Omata,

Iwao Ohtani,

Kohsei Ohtsuki,

Jin OUchi

Publication year - 1978

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/26.4.659836

Subject(s) - osmium tetroxide , glutaraldehyde , electron microscope , rabbit (cipher) , fixative , chemistry , microtome , organ of corti , lactic dehydrogenase , anatomy , chromatography , enzyme , biochemistry , biology , inner ear , pathology , computer science , medicine , physics , cytoplasm , optics , computer security

A method for the detection of lactic dehydrogenase enzymatic activity in outer hair cells of the rabbit is described. The membranous labyrinth with temporal bone was prefixed in glutaraldehyde. After being placed into the incubation medium, it was postfixed in osmium tetroxide. Specimens of the organ of Corti were removed. Then the specimens were embedded in water-soluble glycol and cut with a cryostat for light microscopy, and also they were embedded in Epon and cut for light and electron microscopy. Sectioning of the membranous labyrinth was very easily made when the specimens were embedded in both the water-soluble glycol and the Epon. The structures of the frozen sections as well as the Epon-embedded ones were well preserved. In the frozen sections the preservation and localization of reaction products were thoroughly kept, but monoformazan of the Epon-embedded sections was soluble.

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