Measurement of cellular DNA mass by flow microfluorometry with use of a biological internal standard. | Zendy

Eric Tannenbaum | Zendy; Mary Cassidy | Zendy; Oliver Alabaster | Zendy; C. J. Herman | Zendy

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Measurement of cellular DNA mass by flow microfluorometry with use of a biological internal standard.

Author(s) -

Eric Tannenbaum,

Mary Cassidy,

Oliver Alabaster,

C. J. Herman

Publication year - 1978

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/26.2.624835

Subject(s) - dna , biological materials , chemistry , biological fluids , chromatography , biological system , biology , biochemistry

Use of a biological standard (chicken erythrocytes) mixed with experimental cell populations allows control of all variables in flow microfluorometric determination of DNA content. These variables include both staining and instrument procedures. In addition, the use of a biological standard allows determination of cellular DNA mass in unperturbed cell populations. DNA mass measured by FMF technique correlates closely with values reported in the literature that used biochemical techniques.

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