Immunohistochemical localization of thyroid hormone in rat thyroid gland. | Zendy

Marjorie Wilson | Zendy; Karen R. Hitchcock | Zendy; Ronald A. DeLellis | Zendy

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Immunohistochemical localization of thyroid hormone in rat thyroid gland.

Author(s) -

Marjorie Wilson,

Karen R. Hitchcock,

Ronald A. DeLellis

Publication year - 1978

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/26.12.366016

Subject(s) - thyroid , immunofluorescence , hormone , parathyroid gland , pathology , immunohistochemistry , antiserum , parathyroid hormone , cytoplasm , endocrine gland , biology , medicine , endocrinology , chemistry , antibody , immunology , microbiology and biotechnology , calcium

Direct and indirect immunofluorescence techniques were used to localize the thyroid hormones triidothyronine (T3) and thyroxine (T4) in adult rat thyroid gland. Optimum dilutions of the antisera were established and four tissue fixatives were investigated for usefulness in this technique. Use of antibodies specific for either T3 or T4 resulted in brilliant fluorescence in the colloid pools and apical cytoplasm of follicular cells. In all cases, the adjacent parathyroid gland was devoid of fluorescence. This report demonstrates that these dipeptide hormones can be localized by using immunofluorescence techniques.

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