Strategies for choosing a deoxyribonucleic acid stain for flow cytometry of metaphase chromosomes. | Zendy

Ronald H. Jensen | Zendy; Richard G. Langlois | Zendy; Brian H. Mayall | Zendy

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Strategies for choosing a deoxyribonucleic acid stain for flow cytometry of metaphase chromosomes.

Author(s) -

Ronald H. Jensen,

Richard G. Langlois,

Brian H. Mayall

Publication year - 1977

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/25.8.70463

Subject(s) - chromomycin a3 , ethidium bromide , flow cytometry , metaphase , microbiology and biotechnology , dna , stain , fluorescence , biology , mitosis , aneuploidy , staining , chromosome , chemistry , genetics , chromatin , optics , heterochromatin , gene , physics

Requirements for flow cytometry of metaphase chromosomes stained with three deoxyribonucleic acid (DNA)-specific fluorescent dyes--Hoechst 33258, Chromomycin A3, and ethidium bromide--are reviewed. Fluorescence properties of these three stains when bound to mitotic cells or to chromosomes in suspension are measured and compared with fluorescence properties when bound to DNA in solution. Conditions are given for high resolution flow cytometry of Chinese hamster chromosomes stained with each of the fluorophors, and histograms are presented that exhibit differences in relative peak position and area. Energy transfer fluorescence between two DNA stains is presented as a potentially useful new parameter for flow cytometry of chromosomes and is illustrated by fluorescence energy transfer from Chromomycin A3 to ethidium bromide when simultaneously bound to hamster mitotic cells.

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