A volume-activated cell sorter. | Zendy

E Menke | Zendy; E Kordwig | Zendy; P Stuhlmüller | Zendy; Volker Kachel | Zendy; G. RuhenstrothBauer | Zendy

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A volume-activated cell sorter.

Author(s) -

E Menke,

E Kordwig,

P Stuhlmüller,

Volker Kachel,

G. RuhenstrothBauer

Publication year - 1977

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/25.7.894006

Subject(s) - volume (thermodynamics) , body orifice , detector , electromagnetic shielding , voltage , materials science , electrical engineering , biomedical engineering , engineering , physics , mechanical engineering , quantum mechanics

A sorter that is activated by the resistance pulses of a Coulter orifice (volume detector) has been developed. The problem arising from the distortion of the volume signals produced by the electric droplet forming and charging voltages are avoided by special shielding and grounding of the volume detector. Experiments with beads and leukocytes are described. The maximal processing rate at this time is 2000 cells/sec, the purity of sorted fractions is better than 97%, and the viability of sorted cells is better than 80%.

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