Crypt cell isolation in the small intestine of the mouse. | Zendy

V C Kremski | Zendy; Luca Varani | Zendy; C Desaive | Zendy; Paul S. Miller | Zendy; Claudio Nicolini | Zendy

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Crypt cell isolation in the small intestine of the mouse.

Author(s) -

V C Kremski,

Luca Varani,

C Desaive,

Paul S. Miller,

Claudio Nicolini

Publication year - 1977

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/25.7.894003

Subject(s) - crypt , lumen (anatomy) , isolation (microbiology) , trypsin , small intestine , cell , large intestine , microbiology and biotechnology , chemistry , biology , biochemistry , enzyme , endocrinology

A successful method has been developed for isolating viable single cell suspensions of intestinal crypt cells from the small bowel of the mouse. The lumen of the intestine was perfused with a 0.2% trypsin solution that dissociated the lining epithelial cells. Crypt cell isolation, which proves to be extremely critical, occurred under optimal mechanical and chemical configurations about 75 min after the beginning of the procedure.

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