Differential fluorochromasia of human lymphocytes as measured by flow cytometry. | Zendy

Mark E. Wilder | Zendy; L. Scott Cram | Zendy

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Differential fluorochromasia of human lymphocytes as measured by flow cytometry.

Author(s) -

Mark E. Wilder,

L. Scott Cram

Publication year - 1977

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/25.7.70458

Subject(s) - flow cytometry , fluorescein , staining , fluorescence , microbiology and biotechnology , fluorescent staining , chemistry , differential staining , distribution (mathematics) , cytometry , cell , biology , biochemistry , physics , genetics , mathematical analysis , mathematics , quantum mechanics

Peripheral human lymphocytes reacted with fluorescein diacetate and analyzed by flow cytometry produced a bimodal fluorescence distribution that was shown to be attributable to the differential staining of T and B lymphocytes. Lymphocytes were fractionated into rosetting (T cell) and nonrosetting (B cell) populations. Both subfractions were reacted with fluorescein diacetate and analyzed by flow cytometry. The rosetting fraction was more fluorescent than the nonrosetting fraction, and the analysis of an appropriate mixture of the subfractionated populations produced a fluorescence distribution very similar to that obtained with unfractionated lymphocytes.

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