Two-color immunofluorescence using a fluorescence-activated cell sorter. | Zendy

M R Loken | Zendy; David R. Parks | Zendy; L A Herzenberg | Zendy

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Two-color immunofluorescence using a fluorescence-activated cell sorter.

Author(s) -

M R Loken,

David R. Parks,

L A Herzenberg

Publication year - 1977

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/25.7.330738

Subject(s) - fluorescein , immunofluorescence , fluorescence , rhodamine , rhodamine b , excitation wavelength , antibody , chemistry , materials science , optics , microbiology and biotechnology , biology , immunology , physics , biochemistry , photocatalysis , catalysis

A technique for the analysis of fluorescein and rhodamine in a flow system using a single wavelength of excitation is described. Both optics and electronics are used to discriminate the fluorescein and rhodamine signals. This technique has been used to study the relationship between immunoglobulin M and immunoglobulin D on mouse splenic lymphocytes.

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