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Quantitative extraction of morphologic cell parameters from the diffraction pattern.
Author(s) -
G. Seger,
M. Achatz,
Wiebke Mareile Heinze,
F Sinsel
Publication year - 1977
Publication title -
journal of histochemistry and cytochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.971
H-Index - 124
eISSN - 1551-5044
pISSN - 0022-1554
DOI - 10.1177/25.7.330724
Subject(s) - diffraction , materials science , fourier transform , optics , extraction (chemistry) , fourier analysis , intensity (physics) , amplitude , physics , chemistry , chromatography , quantum mechanics
A new technique for the fast and quantitative extraction of cell parameters from diffraction patterns was applied to cervical gynecologic material. Based on radial scans through the diffraction pattern, this technique permits the simultaneous determination of the nuclear and cytoplasmic diameter by Fourier analysis of the radial scanning signals after compensation for the intensity falloff by an amplification which is proportional to the third power of the radial position in the diffraction plane. In addition, a correlation between the angle of the highest amplitude of the scanning signals and the coarseness of microstructures in the cell was observed. Illustrative examples of measurements on exfoliated cells of different type are presented.

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