Mithramycin fluorescence for quantitative determination of deoxyribonucleic acid in single cells. | Zendy

E. Johannisson | Zendy; B. Thorell | Zendy

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Mithramycin fluorescence for quantitative determination of deoxyribonucleic acid in single cells.

Author(s) -

E. Johannisson,

B. Thorell

Publication year - 1977

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/25.2.138705

Subject(s) - fluorescence , staining , chemistry , dna , fixation (population genetics) , population , cell , reproducibility , fluorescence spectroscopy , lysis , chromatography , microbiology and biotechnology , biochemistry , biology , medicine , genetics , physics , environmental health , quantum mechanics , gene

The use of the antibiotic drug mithramycin for cytoflurometric assessment of deoxyribonucleic acid in single cells has been studied in smears of a standard cell population of rat thymocytes. The optimal staining conditions have been determined including the influence of fixation (freeze-drying, formalin and ethanol). The drug equilibration time has been estimated in relation to the concentration of the mithramycin and to Mg++ was found to enhance the fluorescence intensity produced by the mithramycin-deoxyribonucleic acid interaction. The stability and the reproducibility of the fluorescence reaction are discussed.

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