Retention of the glycocalyx after cell detachment by EGTA. | Zendy

Susan Cox | Zendy; Peter Baur | Zendy; B. R. Haenelt | Zendy

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Retention of the glycocalyx after cell detachment by EGTA.

Author(s) -

Susan Cox,

Peter Baur,

B. R. Haenelt

Publication year - 1977

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/25.12.411829

Subject(s) - glycocalyx , egta , population , ruthenium red , chemistry , biophysics , cell , glycoprotein , microbiology and biotechnology , biochemistry , biology , medicine , calcium , environmental health , organic chemistry

Chinese hamster ovary cells were examined ultrastructurally following several detachment procedures. Alterations in the surface glycoproteins were observed by using ruthenium red in the fixation procedure. Trypsin removed a major portion of the cells glycocalyx and formed spherical cellular configurations. EGTA detached cells were also spherical, however, their glycocalyx appeared to remain although redistributed over the cell surface. Kinetic studies showed no alterations in subsequent population doubling times following either detachment procedure. EGTA may thus represent the current method of choice for cell detachment if preservation of the surface glycoproteins is of interest.

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