Open AccessTwo-wavelength cytophotometry: the choice of the wavelengths from a practical point of view.
Author(s) -
Patrick Van Oostveldt,
Guido Boeken
Publication year - 1977
Publication title -
journal of histochemistry and cytochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.971
H-Index - 124
eISSN - 1551-5044
pISSN - 0022-1554
DOI - 10.1177/25.12.336786
Subject(s) - wavelength , point (geometry) , optics , computer science , physics , mathematics , geometry
Methods in use for the choice of the two-wavelengths and the effect of a faulty choice of the two wavelengths in the two-wavelength cytophotometric method of Patau and Ornstein are studied by means of a computer simulation and in practice upon pararosaniline-Schiff stained nuclei. A simplification of the measuring strategy is proposed without affecting the accuracy.
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