Open AccessTraceable Impedance-Based Dispensing and Cloning of Living Single Cells
Author(s) -
Muller Georges,
Bonzon David,
Claudinot Stéphanie,
Rochat Ariane,
Renaud Philippe,
Barrandon Yann
Publication year - 2020
Publication title -
slas technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.714
H-Index - 16
eISSN - 2472-6311
pISSN - 2472-6303
DOI - 10.1177/2472630320905574
Subject(s) - cloning (programming) , stem cell , isolation (microbiology) , pipette , computational biology , cancer stem cell , biology , microbiology and biotechnology , computer science , chemistry , bioinformatics , programming language
Single-cell cloning is essential in stem cell biology, cancer research, and biotechnology. Regulatory agencies now require an indisputable proof of clonality that current technologies do not readily provide. Here, we report a one-step cloning method using an engineered pipet combined with an impedance-based sensing tip. This technology permits the efficient and traceable isolation of living cells, stem cells, and cancer stem cells that can be individually expanded in culture and transplanted.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom