Novel LC/MS/MS and High-Throughput Mass Spectrometric Assays for Monoacylglycerol Acyltransferase Inhibitors | Zendy

Jenson Qi | Zendy; John A. Masucci | Zendy; Wensheng Lang | Zendy; Margery A. Connelly | Zendy; Gary W. Caldwell | Zendy; Ioanna P. Petrounia | Zendy; Jennifer Kirkpatrick | Zendy; Alexander N. Barnakov | Zendy; Geoffrey T. Struble | Zendy; Robyn Miller | Zendy; Keli Dzordzorine | Zendy; GeeHong Kuo | Zendy; Michael D. Gaul | Zendy; Alessandro Pocai | Zendy; SeungHun Lee | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Novel LC/MS/MS and High-Throughput Mass Spectrometric Assays for Monoacylglycerol Acyltransferase Inhibitors

Author(s) -

Jenson Qi,

John A. Masucci,

Wensheng Lang,

Margery A. Connelly,

Gary W. Caldwell,

Ioanna P. Petrounia,

Jennifer Kirkpatrick,

Alexander N. Barnakov,

Geoffrey T. Struble,

Robyn Miller,

Keli Dzordzorine,

GeeHong Kuo,

Michael D. Gaul,

Alessandro Pocai,

SeungHun Lee

Publication year - 2017

Publication title -

slas discovery

Language(s) - English

Resource type - Journals

eISSN - 2472-5560

pISSN - 2472-5552

DOI - 10.1177/2472555217690101

Subject(s) - monoacylglycerol lipase , diacylglycerol kinase , chemistry , glycerol , chromatography , microsome , biochemistry , mass spectrometry , high throughput screening , tandem mass spectrometry , enzyme , endocannabinoid system , receptor , protein kinase c

Monoacylglycerol acyltransferase enzymes (MGAT1, MGAT2, and MGAT3) convert monoacylglycerol to diacylglycerol (DAG). MGAT1 and MGAT2 are both implicated in obesity-related metabolic diseases. Conventional MGAT enzyme assays use radioactive substrates, wherein the product of the MGAT-catalyzed reaction is usually resolved by time-consuming thin layer chromatography (TLC) analysis. Furthermore, microsomal membrane preparations typically contain endogenous diacylglycerol acyltransferase (DGAT) from the host cells, and these DGAT activities can further acylate DAG to form triglyceride (TG). Our mass spectrometry (liquid chromatography-tandem mass spectrometry, or LC/MS/MS) MGAT2 assay measures human recombinant MGAT2-catalyzed formation of didecanoyl-glycerol from 1-decanoyl-rac-glycerol and decanoyl-CoA, to produce predominantly 1,3-didecanoyl-glycerol. Unlike 1,2-DAG, 1,3-didecanoyl-glycerol is proved to be not susceptible to further acylation to TG. 1,3-Didecanoyl-glycerol product can be readily solubilized and directly subjected to high-throughput mass spectrometry (HTMS) without further extraction in a 384-well format. We also have established the LC/MS/MS MGAT activity assay in the intestinal microsomes from various species. Our assay is proved to be highly sensitive, and thus it allows measurement of endogenous MGAT activity in cell lysates and tissue preparations. The implementation of the HTMS MGAT activity assay has facilitated the robust screening and evaluation of MGAT inhibitors for the treatment of metabolic diseases.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research