Platinum-pyrimidine complexes for electron microscopic cytochemistry of deoxyribonucleic acid. | Zendy

Shruti Aggarwal | Zendy

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Platinum-pyrimidine complexes for electron microscopic cytochemistry of deoxyribonucleic acid.

Author(s) -

Shruti Aggarwal

Publication year - 1976

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/24.9.61242

Subject(s) - cytochemistry , glutaraldehyde , staining , pyrimidine , nucleic acid , nucleolus , chemistry , reagent , platinum , dna , chromatin , nuclear chemistry , biochemistry , biology , chromatography , catalysis , organic chemistry , enzyme , cytoplasm , genetics

Platinum-pyrimidine complexes that are amorphous and highly soluble in water when used as sole electron-dense stains show high selectivity for nucleic acid-rich areas like chromatin, nucleolus and ribosomes. A method is presented for the selective staining of deoxyribonucleic acid. Glutaraldehyde-fixed tissue are exposed to 3 N HCL hydrolysis for an optimum time of 1 hr at room temperature before being embedded in Epon. Thin sections are then exposed to Schiff's reagent for 30 min and treated with 1% platinum-pyrimidine complex. The results inselective staining of structures containing deoxyribonucleic acid.

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