Biological strandard for electron microprobe analysis of intracellular sodium concentration. | Zendy

Vikrom S. Sottiurai | Zendy; Richard L. Malvin | Zendy; Lawrence F. Allard | Zendy; W. C. Bigelow | Zendy

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Biological strandard for electron microprobe analysis of intracellular sodium concentration.

Author(s) -

Vikrom S. Sottiurai,

Richard L. Malvin,

Lawrence F. Allard,

W. C. Bigelow

Publication year - 1976

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/24.6.985836

Subject(s) - fixative , electron microprobe , intracellular , flame photometry , sodium , chemistry , photometry (optics) , chromatography , electron probe microanalysis , biophysics , saline , analytical chemistry (journal) , mineralogy , biochemistry , biology , physics , organic chemistry , cytoplasm , stars , astronomy , endocrinology

A method is described for the determination of the intracellular concentration of sodium in individual cells using the electron microprobe analyzer. This method gives an accuracy equal to that obtained by using flame photometry on tissues with large cell populations. Intracellular sodium was precipitated in the cell by a fixative containing pyroantimonate. Cartilaginous needles from shark fins which were equilibrated in saline solutions of differing concentrations were used as biological standards.

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