Dimethyl sulfoxide-lead tetraacetate method for histochemical oxidation of polysaccharides. | Zendy

George I. Malinin | Zendy

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Dimethyl sulfoxide-lead tetraacetate method for histochemical oxidation of polysaccharides.

Author(s) -

George I. Malinin

Publication year - 1976

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/24.2.56394

Subject(s) - dimethyl sulfoxide , reagent , chemistry , sodium bisulfite , polysaccharide , staining , toluidine , glycogen , nuclear chemistry , organic chemistry , biochemistry , biology , genetics

Oxidation of fixed tissues and unfixed peripheral blood smears by 1% (w/v) lead tetraacetate in dimethyl sulfoxide for 10 to 60 min resulted in facile induction of tissue carbonyls readily demonstrable with Schiff's reagent and by sodium bisulfite addition reaction, followed by toluidine blue staining at controlled pH. Tissue carbonyls represented a broad range of oxidation-labile substrates and included neutral polysaccharides (glycogen). The oxidation procedures were not destructive to tissues and were characterized by technical simplicity and consistent reproducibility, thus affording a substantial improvement over the hitherto used methods of histochemical oxidation by lead tetraacetate.

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