A microspectrophotometric study of Papanicolaou-stained cervical cells as an aid in computerized image processing. | Zendy

J Holmquist | Zendy; Y Imasoto | Zendy; Ewert Bengtsson | Zendy; Bengt Olsen | Zendy; Björn Stenkvist | Zendy

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A microspectrophotometric study of Papanicolaou-stained cervical cells as an aid in computerized image processing.

Author(s) -

J Holmquist,

Y Imasoto,

Ewert Bengtsson,

Bengt Olsen,

Björn Stenkvist

Publication year - 1976

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/24.12.63509

Subject(s) - papanicolaou stain , minicomputer , cytoplasm , photometer , cytological techniques , nucleus , pathology , biology , optics , medicine , computer science , physics , microbiology and biotechnology , cervical cancer , genetics , cancer , operating system

As part of a study of cytologic automation, microspectrophotometric investigation of Papanicolaou-stained cervical cells was performed, using a Leitz MPV-II scanning photometer connected to a PDP 8/F minicomputer. It was shown that the selection of one single wavelength may result in difficulties in detecting boundries between background and cytoplasm and/or between cytoplasm nucleus. A set of two wavelengths, 530 nm and 570 nm, were found to be optimal for the image processing of these cells.

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