Use of actinomycin D for the specific quenching of fluorescence of deoxyribonucleic acid in cells stained with acridine aminoderivatives. | Zendy

A. V. Zelenin | Zendy; E. A. Kirianova | Zendy; V. A. Kolesnikov | Zendy; N. G. Stepanova | Zendy

Open Access

Use of actinomycin D for the specific quenching of fluorescence of deoxyribonucleic acid in cells stained with acridine aminoderivatives.

Author(s) -

A. V. Zelenin,

E. A. Kirianova,

V. A. Kolesnikov,

N. G. Stepanova

Publication year - 1976

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/24.11.63505

Subject(s) - acridine orange , dna , fluorescence , rna , acridine , chemistry , nucleic acid , biochemistry , quenching (fluorescence) , microbiology and biotechnology , biology , organic chemistry , apoptosis , physics , gene , quantum mechanics

Actinomycin D specifically quenches the fluorescence of acridine orange and quinacrine bound to deoxyribonucleic acid in cytologic preparations, but does not change the fluorescence of these fluorochromes bound to RNA. The following fluorescence-cytochemical applications of techniques based on these findings can be suggested: (a) distinction between deoxyribonucleic acid and ribonucleic acid; (b) detection of double-stranded virus ribonucleic acid; (c) approximate estimation of the lengths of A-T sequences in deoxyribonucleic acid molecules.

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