A comparative ultrastructural localization of concanavalin A, wheat germ and Ricinus communis on glomeruli of normal rat kidney. | Zendy

R Bretton | Zendy; J Bariéty | Zendy

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A comparative ultrastructural localization of concanavalin A, wheat germ and Ricinus communis on glomeruli of normal rat kidney.

Author(s) -

R Bretton,

J Bariéty

Publication year - 1976

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/24.10.977938

Subject(s) - concanavalin a , ricinus , wheat germ agglutinin , endoplasmic reticulum , glomerulus , lectin , basement membrane , biology , peroxidase , golgi apparatus , ultrastructure , glomerular basement membrane , microbiology and biotechnology , chemistry , biochemistry , kidney , anatomy , glomerulonephritis , enzyme , endocrinology , in vitro

Three lectins, concanavalin A, peroxidase labeled wheat germ and peroxidase labeled Ricinus communis have been utilized to determine the localization of various saccharide determinants in the glomerulus of the normal rat kidney. In order to obtain a homogeneous penetration of the lectins throughout the whole section, various technical parameters were studied. Only with concanavalin A, a diffuse labeling of the endoplasmic reticulum was obtained. With the three lectins, the basement membrane, mesangial matrix and the cell coat of the three cell types in the glomerulus were labeled. Differences in the labeling of the capillary wall were also noted.

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