Plasma protein immunocytochemistry and polysaccharide cytochemistry at the surface of alveolar and endothelial cells in the rat lung.
Author(s) -
J Big,
Francis Jaubert,
MarieClaude Jaurand
Publication year - 1976
Publication title -
journal of histochemistry and cytochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.971
H-Index - 124
eISSN - 1551-5044
pISSN - 0022-1554
DOI - 10.1177/24.10.789758
Subject(s) - cytochemistry , immunocytochemistry , ultrastructure , lung , concanavalin a , pathology , chemistry , endothelial stem cell , alveolar epithelium , cell , epithelium , microbiology and biotechnology , biology , biochemistry , anatomy , medicine , in vitro
Ultrastructural immunocytochemistry of plasma proteins and cytochemistry of polysaccharides with ruthenium red and concanavalin A were combined with different types of fixation with or without prior vascular or airway washing, to study the surface of capillary endothelial and alveolar epithelial cells of the blood-air barrier in the rat lung. The endothelial and epithelial cell surface layers were found to have two components: a moveable part belonging to the cellular microenvironement made of plasma proteins and a deeper glucidic or anionic fixed part bound to the plasma membranes (cell coat).
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