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Gynecologic specimen analysis by multiangle light scattering in a flow system.
Author(s) -
G.C. Salzman,
J. M. Crowell,
K. M. Hansen,
Melissa Ingram,
P. F. Mullaney
Publication year - 1976
Publication title -
journal of histochemistry and cytochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.971
H-Index - 124
eISSN - 1551-5044
pISSN - 0022-1554
DOI - 10.1177/24.1.943440
Subject(s) - optics , light scattering , laser beams , laser , laser light , scattering , light beam , flow (mathematics) , multiangle light scattering , beam (structure) , materials science , physics , mechanics
A flow-system instrument is described in which the laser light scattered by a mammalian cell is sampled simultaneously at up to 32 angles between 0 degrees and 21 degrees from the laser beam axis as the cell passes through the beam. The scatter pattern for each cell is stored by a computer for later analysis. Various data-processing techniques are discussed. Results of preliminary application of the instrument to the analysis of normal and abnormal gynecologic specimens are presented.

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