Assessment of monocyte esterase activity by flow cytophotometry. | Zendy

Leonard S. Kaplow | Zendy; H Dauber | Zendy; E Lerner | Zendy

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Assessment of monocyte esterase activity by flow cytophotometry.

Author(s) -

Leonard S. Kaplow,

H Dauber,

E Lerner

Publication year - 1976

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/24.1.1254930

Subject(s) - esterase , staining , stain , intracellular , flow cytometry , population , monocyte , chemistry , suspension (topology) , microbiology and biotechnology , biochemistry , enzyme , biology , immunology , medicine , genetics , mathematics , environmental health , homotopy , pure mathematics

An azo dye supravital method has been devised for selectively staining human monocytes in suspension for nonspecific esterase activity. Stained cells can be identified and rapidly enumerated by presenting the suspension of stained cells to the Cytograf, a flow-through cell discriminating cytophotometer. The intensity of stain is proportional to the intracellular esterase activity. By analysis of the oscilloscope display, it has been possible to obtain relative data concerning the degree of activity of monocyte nonspecific esterase activity. These observations suggest a unique approach to the measurement of intracellular enzyme activity in selected cells in a mixed population.

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