Open AccessTime Required for Effective Action of Phenol against Giant Cell Tumour Cells
Author(s) -
Manasseh Nithyananth,
Anne Jennifer Priscilla,
P V J R C Boopalan,
V. T. K. Titus,
Ver N. Lee
Publication year - 2014
Publication title -
journal of orthopaedic surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.457
H-Index - 40
eISSN - 2309-4990
pISSN - 1022-5536
DOI - 10.1177/230949901402200126
Subject(s) - medicine , phenol , giant cell , penetration (warfare) , programmed cell death , cell , tibia , surgery , pathology , apoptosis , biochemistry , biology , chemistry , organic chemistry , operations research , engineering
Purpose. To evaluate the time required for effective action of phenol against the giant cell tumour (GCT) cells.Methods. Fresh GCT cells were harvested from 9 patients with primary GCT of the distal femur (n=4), proximal tibia (n=4), and proximal humerus (n=1), with the Campanacci tumour grades 3 (n=6), 2 (n=2), and 1 (n=1). Specimens were immersed in 80 % phenol for one, 3, 6, and 10 minutes, and were assessed by a single pathologist for irreversible cell death and the depth of phenol penetration.Results. Phenol caused consistent GCT cell death in 6 of the 9 specimens after 3 minutes and in all 9 specimens after 6 minutes, compared to none in controls (p<0.0001). The mean depths of phenol penetration were 15 (range, 11–20) and 19 (range, 15–25) cell thickness after 6 and 10 minutes, respectively (p<0.0001).Conclusion. GCT cells immersed in 80% phenol for 6 minutes resulted in consistent cell death.