Diaminobenzidine reactivity of mitochondria and peroxisomes in Tetrahymena and in wild-type and cytochrome oxidase-deficient Paramecium. | Zendy

Nicole Stelly | Zendy; M Balmefrézol | Zendy; A Adoutte | Zendy

Open Access

Diaminobenzidine reactivity of mitochondria and peroxisomes in Tetrahymena and in wild-type and cytochrome oxidase-deficient Paramecium.

Author(s) -

Nicole Stelly,

M Balmefrézol,

A Adoutte

Publication year - 1975

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/23.9.170331

Subject(s) - tetrahymena , cytochrome c oxidase , paramecium aurelia , peroxisome , paramecium , tetrahymena pyriformis , mitochondrion , biology , biochemistry , oxidase test , organelle , cytochrome , horseradish peroxidase , microbiology and biotechnology , chemistry , enzyme , gene

Reactivity of mitochondria and peroxisomes to diaminobenzidine was investigated in Tetrahymena pyriformis and in wild-type and cytochrome oxidase-deficient Paramecium aurelia. Wild-type and cytochrome oxidase-deficient Paramecium gave positive mitochondrial reactions in the absence of added H2O2, and the deposits were enhanced by the addition of H2O2, whereas Tetrahymena gave positive mitochondrial reactions only upon addition of H2O2. These results are discussed in the light of the current ideas concerning the mechanism of staining by diaminobenzidine. Peroxisome-like organelles which react positively to diaminobenzidine, the reaction being partially inhibited by aminotriazole, were identified in both protozoa.

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