Adenylate deaminase. A method for its localization in skeletal muscle. | Zendy

K. Nakatsu | Zendy

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Adenylate deaminase. A method for its localization in skeletal muscle.

Author(s) -

K. Nakatsu

Publication year - 1975

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/23.3.47868

Subject(s) - amp deaminase , adenylate kinase , skeletal muscle , chemistry , enzyme , biochemistry , adenosine deaminase , biophysics , anatomy , biology

A method for light microscopic localization of adenylate deaminase in sections of frozen rat quadriceps muscle is described. The method depends on the hydrolysis of 6-chloropurine ribonucleotide (the 6-chloroanalogue of adenylate) and the trapping of Cl minus by Ag plus. The resulting AgCl precipitate was made visible by exposing the sections to light. After this treatment black deposits about 1 mu in diameter were seen in muscle cells. These observations indicate that adenylate deaminase of rat quadriceps muscle is located at discrete sites within the muscle cells.

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