Quantitation of isolated rat islets of Langerhans on the basis of deoxyribonucleic acid content under metabolic conditions of altered protein synthesis. | Zendy

Adibah Parman | Zendy

Open Access

Quantitation of isolated rat islets of Langerhans on the basis of deoxyribonucleic acid content under metabolic conditions of altered protein synthesis.

Author(s) -

Adibah Parman

Publication year - 1975

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/23.3.1092753

Subject(s) - islet , dna , hypophysectomy , endocrinology , medicine , chemistry , biology , dry weight , wet weight , biochemistry , hormone , insulin , botany

A micromethod was employed to estimate quantitatively and reproducibly the deoxyribonucleic acid (DNA) content of isolated rat islets of Langerhans. The DNA content per islet varied linearly with the mean diameter or the dry weight of the islets isolated. The DNA in the freeze-dried islets of male Sprague-Dawley or Wistar rats was about 21.0 mug/mug islet dry weight. Three to four weeks after hypophysectomy, with or without short term bovine growth hormone replacement, the DNA content per unit dry weight of islets was not significantly altered. Islet DNA content and islet dry weight are proposed as an interconvertible and reliable basis of reference for measurements of different islet functions.

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