Rapid chemical dehydration of samples for electron microscopic examinations. | Zendy

Linda L. Muller | Zendy; T. J. Jacks | Zendy

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Rapid chemical dehydration of samples for electron microscopic examinations.

Author(s) -

Linda L. Muller,

T. J. Jacks

Publication year - 1975

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/23.2.1117127

Subject(s) - acetone , ultrastructure , dehydration , chemistry , chromatography , electron microscope , cotyledon , nuclear chemistry , biochemistry , botany , biology , physics , optics

Acidified 2,2-dimethoxypropane was used to chemically dehydrate biologic tissues for examination in the electron microscope. The ultrastructural integrities of single-celled algae, plant tissues (cotyledon, root, leaf) and animal tissues (liver, pancreas, muscle, cartilage) were maintained. Our technique was simpler and quicker than physically exchanging water for organic solvents (e.g., acetone, ethanol) as generally performed in microscopy.

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