Improving Laboratory Efficiency by Automation of Preanalytic Processing of ThinPrep Specimens for Real-Time PCR High-Risk HPV Testing | Zendy

Daniela Barbieri | Zendy; Simona Venturoli | Zendy; Silvano Costa | Zendy; Maria Paola Landini | Zendy

Open Access

Improving Laboratory Efficiency by Automation of Preanalytic Processing of ThinPrep Specimens for Real-Time PCR High-Risk HPV Testing

Author(s) -

Daniela Barbieri,

Simona Venturoli,

Silvano Costa,

Maria Paola Landini

Publication year - 2015

Publication title -

slas technology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.714

H-Index - 16

eISSN - 2472-6311

pISSN - 2472-6303

DOI - 10.1177/2211068215569347

Subject(s) - computer science , reproducibility , laboratory automation , concordance , vial , sample (material) , spotting , protocol (science) , repeatability , automated method , automation , medicine , chromatography , pathology , artificial intelligence , engineering , chemistry , mechanical engineering , alternative medicine

Cervical specimens collected in liquid-based cytology (LBC) media are the most common sample type used for high-risk human papillomavirus (HPV) testing. Since preanalytic steps such as vortexing and decapping vials, liquid transfer to a sample input tube with matching unique identifier, and recapping the original vials are required for processing LBC samples prior to running the Abbott RealTime High Risk HPV assay (Abbott, Wiesbaden, Germany), a full manual execution can be complicated, especially in high-throughput diagnostic contexts. Here, a custom-configured worktable setup for the Tecan Freedom EVO (Tecan, Männedorf, Switzerland) designed to automate and control preanalytic steps for ThinPrep (Hologic, Marlborough, MA) samples was used to evaluate the impact of automated versus manual preanalytics. Archival results for manual processing of 226 samples were compared with those obtained with the Tecan protocol, observing a very good overall concordance for final assay interpretation (95.6%). High overall agreement (100%) resulted also from retesting 99 samples by both the preanalytical protocols. High reproducibility was observed analyzing 23 randomly selected samples by automated preprocessing in triplicate. Hence, the new configuration of the Tecan platform translates the manual steps required to process ThinPrep specimens into automated operations, controls sample identification, and allows for saving hands-on time, while maintaining assay reproducibility and ensuring reliability of results, making it suitable for screening settings.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research