VISUALIZATION OF DEOXYRIBONUCLEIC ACID MOLECULES BY PROTEIN FILM ADSORPTION AND TANTALUM-TUNGSTEN SHADOWING | Zendy

R. Abermann | Zendy; Miriam M. Salpeter | Zendy

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VISUALIZATION OF DEOXYRIBONUCLEIC ACID MOLECULES BY PROTEIN FILM ADSORPTION AND TANTALUM-TUNGSTEN SHADOWING

Author(s) -

R. Abermann,

Miriam M. Salpeter

Publication year - 1974

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/22.9.845

Subject(s) - tantalum , tungsten , dna , molecule , monolayer , chemistry , substrate (aquarium) , adsorption , electron microscope , crystallography , biophysics , materials science , nanotechnology , biochemistry , optics , biology , organic chemistry , ecology , physics

Preparatory procedures and high resolution shadowing of deoxyribonucleic acid (DNA) molecules for electron microscopy are described. They involve a two-stage mounting of the DNA on cytochrome c monolayers and shadowing with tantalum-tungsten. Conditions are determined that produce a smooth substrate and reproducible binding of the DNA strands in a randomly coiled manner without obvious stretching artifacts. When limiting factors such as excess buffer salts are eliminated, the DNA strands have the over-all dimensions expected from the naked molecule.

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