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A method is presented for the computer synthesis of deoxyribonucleic acid (DNA) histograms, based on estimates of cell distribution around a life cycle, DNA synthesis rate and instrumentally introduced dispersion. Synthetic histograms are compared with experimental DNA histograms for two cell systems; in one, the cell population is in asynchronous exponential growth (steady state), in the other, the cell population is recovering from a block at the G 1 /S phase interface (nonsteady state). The average phase transit times and the associated dispersions are determined, using a DNA histogram synthesis technique, for the nonsteady state population.

CELL CYCLE ANALYSIS FROM COMPUTER SYNTHESIS OF DEOXYRIBONUCLEIC ACID HISTOGRAMS