LOCALIZATION OF α-FETOPROTEIN IN HUMAN AND RAT FETAL LIVERS AN IMMUNOHISTOCHEMICAL METHOD USING HORSERADISH PEROXIDASE | Zendy

N C Nayak | Zendy; P. K. Das | Zendy; U N Bhuyan | Zendy; Asha Mittal | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

LOCALIZATION OF α-FETOPROTEIN IN HUMAN AND RAT FETAL LIVERS AN IMMUNOHISTOCHEMICAL METHOD USING HORSERADISH PEROXIDASE

Author(s) -

N C Nayak,

P. K. Das,

U N Bhuyan,

Asha Mittal

Publication year - 1974

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/22.6.414

Subject(s) - immunoperoxidase , horseradish peroxidase , immunohistochemistry , immunofluorescence , efferent , pathology , peroxidase , cytoplasm , connective tissue , fetus , biology , chemistry , anatomy , antibody , microbiology and biotechnology , medicine , biochemistry , enzyme , immunology , monoclonal antibody , pregnancy , genetics , afferent

α-Fetoprotein (AFP) was successfully demonstrated in paraffin-embedded sections of human and rat fetal livers, by a multilayering immunohistochemical technique using the peroxidase-antiperoxidase system. Serial sections simultaneously subjected to immunofluorescence showed identical sites of localization in hepatocytic cytoplasm and sometimes in perivenous connective tissue. AFP-containing hepatocytes were located around efferent veins as well as randomly in the lobule. The immunoperoxidase technique has certain advantages over the immunofluorescence method which would justify the former's application in studies on the dynamics of AFP synthesis.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research