REFINEMENT OF THE BIS-(THIOACETOXY) AURATE (I) METHOD FOR THE ELECTRON MICROSCOPIC LOCALIZATION OF ACETYLCHOLINESTERASE AND NONSPECIFIC CHOLINESTERASE | Zendy

George B. Koelle | Zendy; Richard W. Davis | Zendy; Eloise Gabel Smyrl | Zendy; ASHLEY V. FINE | Zendy

Open Access

REFINEMENT OF THE BIS-(THIOACETOXY) AURATE (I) METHOD FOR THE ELECTRON MICROSCOPIC LOCALIZATION OF ACETYLCHOLINESTERASE AND NONSPECIFIC CHOLINESTERASE

Author(s) -

George B. Koelle,

Richard W. Davis,

Eloise Gabel Smyrl,

ASHLEY V. FINE

Publication year - 1974

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/22.4.252

Subject(s) - acetylcholinesterase , electron microscope , cholinesterase , resolution (logic) , biophysics , chemistry , anatomy , pathology , enzyme , biology , biochemistry , physics , optics , computer science , medicine , endocrinology , artificial intelligence

The bis-(thioacetoxy) aurate (I) histochemical method has been refined to permit reliable electron microscopic localization of acetylcholinesterase and nonspecific cholinesterase in autonomic ganglia and other mammalian and submammalian tissues. The detailed procedure is presented, along with illustrations of its specificity by light microscopy and high degree of resolution by electron microscopy.

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