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The immoperoxidase-immunoglobulin bridge technique has been utilized for the localization of lysozyme (LZM) in the cells and tissue of the normal rat. Specific LZM staining was found in the proximal tubules of the kidney, Paneth cells of the small intestine and alveolar macrophages. LZM staining was also demonstrated in macrophages in the perifollicular sinusoids of an activated lymph node. The immunoperoxidase method appears to have significant advantages over previously described methods for LZM localization, and it should also be adaptable to electron microscopic studies.

LOCALIZATION OF LYSOZYME IN NORMAL RAT TISSUES BY AN IMMUNOPEROXIDASE METHOD