LOCALIZATION OF LYSOZYME IN NORMAL RAT TISSUES BY AN IMMUNOPEROXIDASE METHOD | Zendy

Matti Klockars | Zendy; Elliott F. Osserman | Zendy

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LOCALIZATION OF LYSOZYME IN NORMAL RAT TISSUES BY AN IMMUNOPEROXIDASE METHOD

Author(s) -

Matti Klockars,

Elliott F. Osserman

Publication year - 1974

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/22.3.139

Subject(s) - immunoperoxidase , lysozyme , staining , pathology , lymph node , immunohistochemistry , chemistry , antibody , biology , medicine , biochemistry , immunology , monoclonal antibody

The immoperoxidase-immunoglobulin bridge technique has been utilized for the localization of lysozyme (LZM) in the cells and tissue of the normal rat. Specific LZM staining was found in the proximal tubules of the kidney, Paneth cells of the small intestine and alveolar macrophages. LZM staining was also demonstrated in macrophages in the perifollicular sinusoids of an activated lymph node. The immunoperoxidase method appears to have significant advantages over previously described methods for LZM localization, and it should also be adaptable to electron microscopic studies.

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