PEROXIDASE-LABELED ANTIBODY A NEW METHOD OF CONJUGATION | Zendy

Paul K. Nakane | Zendy; Akira Kawaoi | Zendy

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PEROXIDASE-LABELED ANTIBODY A NEW METHOD OF CONJUGATION

Author(s) -

Paul K. Nakane,

Akira Kawaoi

Publication year - 1974

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/22.12.1084

Subject(s) - peroxidase , sodium periodate , horseradish peroxidase , chemistry , periodate , biochemistry , moiety , enzyme , aldehyde , antibody , chromatography , stereochemistry , organic chemistry , biology , immunology , catalysis

A new method of conjugating horseradish peroxidase with proteins was developed. The carbohydrate moiety of fluorodinitrobenzene-blocked peroxidase was oxidized with sodium periodate to form aldehyde groups. The peroxidase-aldehyde was then bound to free amino groups of proteins unidirectionally at high efficiencies. Peroxidase-labeled immunoglobulin retained its immunologic as well as enzymatic activities.

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