HUMAN LEUKEMIC CELLS CYTOCHEMICAL STUDIES ON ACIDIC NUCLEAR PROTEINS | Zendy

L.S. Desai | Zendy; G. E. Foley | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

HUMAN LEUKEMIC CELLS CYTOCHEMICAL STUDIES ON ACIDIC NUCLEAR PROTEINS

Author(s) -

L.S. Desai,

G. E. Foley

Publication year - 1974

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/22.1.40

Subject(s) - nucleolus , nuclear protein , nuclear dna , staining , dna , cell nucleus , chemistry , microbiology and biotechnology , toluidine , biochemistry , biology , cytoplasm , gene , genetics , transcription factor , mitochondrial dna

Utilizing staining with alkaline fast green (pH 8.1) and toluidine blue O (pH 9.0) stains, the nuclear basic and acidic protein content of individual human lymphocytic cells has been quantitated cytochemically with high resolution, rapid scanning instruments. There were distinct differences in the distribution patterns of the acidic and basic proteins in these human lymphocytic cells. Nucleoli were densely stained by toluidine blue O, confirming the presence of acidic proteins. The acidic nuclear proteins:DNA and basic nuclear proteins:DNA ratios are consistent and quantitatively similar, irrespective of the diagnostic category from which the cells derived and there was a definite correlation between nuclear proteins and DNA content. However, the acidic nuclear proteins represented a larger proportion of the total dry mass of the cell than did the basic nuclear proteins, an observation which may prove to be of interest with respect to control mechanisms.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research