Expression and significance of SDF-1 and its receptor CXCR4 in the retina of pregnant rats after optic nerve injury | Zendy

Tiegang Liu | Zendy; Xin Tang | Zendy; Ling Yao | Zendy; Hui Song | Zendy

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Expression and significance of SDF-1 and its receptor CXCR4 in the retina of pregnant rats after optic nerve injury

Author(s) -

Tiegang Liu,

Xin Tang,

Ling Yao,

Hui Song

Publication year - 2018

Publication title -

european journal of inflammation

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.219

H-Index - 20

eISSN - 2058-7392

pISSN - 1721-727X

DOI - 10.1177/2058739218819675

Subject(s) - retina , stromal cell , cxcr4 , optic nerve , immunofluorescence , pathology , western blot , immunohistochemistry , retinal , medicine , biology , andrology , receptor , immunology , anatomy , ophthalmology , antibody , chemokine , neuroscience , biochemistry , gene

Stromal cell–derived factor 1 (SDF-1) and its receptor CXCR4 have shown to play a role in embryonic development process, regulation of hematopoiesis, mediating immunology response, inflammatory reaction, and metastasis of malignant tumor. Recently, SDF-1 and CXCR4 are also closely related to retinal neovascularization. This study was to investigate the expression of SDF-1 and CXCR4 in the retina after optic nerve injury in pregnant rats so as to reveal its significance. A total of 12 pregnant rats were randomly divided into normal group and experimental group (after 5 days of optic nerve injury), six rats in each group; expressions of SDF-1 as well as CXCR4 in rat retina were detected by immunofluorescence staining and western blot assay. The result of immunofluorescence staining showed that the relative gray scale values of SDF-1 and CXCR4 in the experimental group were significantly higher than those in the normal group ( P < 0.05), and the result of Western blot assay showed that the expression levels of SDF-1 and CXCR4 in the experimental group were significantly higher than those in the normal group ( P < 0.05). In conclusion, SDF-1 and its receptor CXCR4 have abnormal expression in the retina of pregnant rats after optic nerve injury, which may be involved in the occurrence and development of optic nerve injury.

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