Activation of RAW264.7 cells by PCp-I, a polysaccharide from Psoralea corylifolia L, through NF-κB/MAPK signalling pathway | Zendy

Honglin Wang | Zendy; Xiaoqing Xu | Zendy; Zhenhua Yin | Zendy; Mengke Wang | Zendy; Baoguang Wang | Zendy; Changyang Ma | Zendy; Jinmei Wang | Zendy; Wenyi Kang | Zendy

Open Access

Activation of RAW264.7 cells by PCp-I, a polysaccharide from Psoralea corylifolia L, through NF-κB/MAPK signalling pathway

Author(s) -

Honglin Wang,

Xiaoqing Xu,

Zhenhua Yin,

Mengke Wang,

Baoguang Wang,

Changyang Ma,

Jinmei Wang,

Wenyi Kang

Publication year - 2021

Publication title -

international journal of immunopathology and pharmacology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.724

H-Index - 53

eISSN - 2058-7384

pISSN - 0394-6320

DOI - 10.1177/20587384211010058

Subject(s) - psoralea corylifolia , mapk/erk pathway , p38 mitogen activated protein kinases , microbiology and biotechnology , chemistry , tlr4 , phosphorylation , signal transduction , nf κb , biochemistry , biology , medicine , alternative medicine , pathology

PCp-I is a polysaccharide isolated and identified from the Psoralea corylifolia L. by our research group. In this study, the immunomodulatory effects of PCp-I on RAW264.7 cells was evaluated. PCp-I could enhance the level of NO along with up-regulation of iNOS mRNA in RAW264.7 cells. The PCp-I could significantly up-regulate the mRNA expression of TNF- α and IL-6 in RAW264.7 cells, and then the expression of TNF- α, IL-6, ROS and the phagocytic activity were increased. Additionally, PCp-I could significantly up-regulate the phosphorylation level of p65, p38, ERK and JNK proteins, which proved that PCp-I could activate the macrophages by MAPKs and NF- κB signalling pathway and the TLR4 may be one of the receptors of PCp-I regulate the RAW264.7 cells.

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