Open AccessPreservation of the naïve features of mesenchymal stromal cells in vitro: Comparison of cell- and bone-derived decellularized extracellular matrix
Author(s) -
Ana Rita Pereira,
Drenka Trivanović,
Philipp Stahlhut,
Maximilian Rudert,
Jürgen Gröll,
Marietta Herrmann
Publication year - 2022
Publication title -
journal of tissue engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.436
H-Index - 30
ISSN - 2041-7314
DOI - 10.1177/20417314221074453
Subject(s) - decellularization , mesenchymal stem cell , stromal cell , microbiology and biotechnology , extracellular matrix , in vitro , bone marrow , chemistry , scaffold , stem cell , matrix (chemical analysis) , cell , biology , biomedical engineering , immunology , cancer research , biochemistry , medicine , chromatography
The fate and behavior of bone marrow mesenchymal stem/stromal cells (BM-MSC) is bidirectionally influenced by their microenvironment, the stem cell niche, where a magnitude of biochemical and physical cues communicate in an extremely orchestrated way. It is known that simplified 2D in vitro systems for BM-MSC culture do not represent their naïve physiological environment. Here, we developed four different 2D cell-based decellularized matrices (dECM) and a 3D decellularized human trabecular-bone scaffold (dBone) to evaluate BM-MSC behavior. The obtained cell-derived matrices provided a reliable tool for cell shape-based analyses of typical features associated with osteogenic differentiation at high-throughput level. On the other hand, exploratory proteomics analysis identified native bone-specific proteins selectively expressed in dBone but not in dECM models. Together with its architectural complexity, the physico-chemical properties of dBone triggered the upregulation of stemness associated genes and niche-related protein expression, proving in vitro conservation of the naïve features of BM-MSC.