IDENTIFICATION OF PEROXISOMES IN THE RAT ADRENAL CORTEX
Author(s) -
Margaret E. Beard
Publication year - 1972
Publication title -
journal of histochemistry and cytochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.971
H-Index - 124
eISSN - 1551-5044
pISSN - 0022-1554
DOI - 10.1177/20.3.173
Subject(s) - peroxisome , organelle , microbody , cytochemistry , zona fasciculata , zona reticularis , ultrastructure , staining , acid phosphatase , biochemistry , adrenal cortex , biology , lipofuscin , cortex (anatomy) , chemistry , microbiology and biotechnology , anatomy , enzyme , zona glomerulosa , genetics , receptor , neuroscience , gene , angiotensin ii
Organelles with the ultrastructure and cytochemical characteristics of peroxisomes (microbodies) have been identified in cells of the zona fasciculata and zona reticularis of the rat adrenal cortex. These peroxisomes appear as small, elliptical to spherical or branched structures, enclosed by a single membrane and composed of a moderately electron-dense matrix. They do not possess a nucleoid or core of the type found in peroxisomes of liver and kidney. These organelles show a strongly positive staining reaction with the diaminobenzidine technique for peroxidatic activity of catalase. This staining is inhibited by aminotriazole. In cytochemical preparations revealing acid phosphatase activity, lysosomes are strongly stained and peroxisomes are free of reaction product.
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