English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

The physical and chemical properties of fluorescein isothiocyanate- and dansylchloridelabeled immunoglobulins were investigated. A marked change in solubility after labeling was noted. Depending on the time and on the type of fluorescent labeling rabbit and bovine immunoglobulin G showed different tendencies for precipitate formation. Increasing the molar fluorescein-protein ratio as well as lowering the pH of the conjugate solutions caused pronounced insolubilization. An increase in the NaCl concentration up to 0.5 M had no significant effect on precipitate formation. A simple method for the elimination of nonspecific staining is given. The method is based on the fact that overlabeled components of fluorescein isothiocyanate conjugates tend to precipitate; this can be initiated and furthered by lowering the pH of the conjugate solution.

CHANGES IN THE SOLUBILITY OF IMMUNOGLOBULINS AFTER FLUORESCENT LABELING AND ITS INFLUENCE ON IMMUNOFLUORESCENT TECHNIQUES