CHANGES IN THE SOLUBILITY OF IMMUNOGLOBULINS AFTER FLUORESCENT LABELING AND ITS INFLUENCE ON IMMUNOFLUORESCENT TECHNIQUES | Zendy

W. Arnold | Zendy; H Mayersbach | Zendy

Open Access

CHANGES IN THE SOLUBILITY OF IMMUNOGLOBULINS AFTER FLUORESCENT LABELING AND ITS INFLUENCE ON IMMUNOFLUORESCENT TECHNIQUES

Author(s) -

W. Arnold,

H Mayersbach

Publication year - 1972

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1177/20.12.975

Subject(s) - conjugate , fluorescein , fluorescein isothiocyanate , solubility , chemistry , fluorescence , isothiocyanate , chromatography , molar ratio , staining , aqueous solution , antibody , biophysics , biochemistry , organic chemistry , biology , catalysis , mathematical analysis , physics , mathematics , quantum mechanics , immunology , genetics

The physical and chemical properties of fluorescein isothiocyanate- and dansylchloridelabeled immunoglobulins were investigated. A marked change in solubility after labeling was noted. Depending on the time and on the type of fluorescent labeling rabbit and bovine immunoglobulin G showed different tendencies for precipitate formation. Increasing the molar fluorescein-protein ratio as well as lowering the pH of the conjugate solutions caused pronounced insolubilization. An increase in the NaCl concentration up to 0.5 M had no significant effect on precipitate formation. A simple method for the elimination of nonspecific staining is given. The method is based on the fact that overlabeled components of fluorescein isothiocyanate conjugates tend to precipitate; this can be initiated and furthered by lowering the pH of the conjugate solution.

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